Technical Note: #9 Bradford Assay in Microvolume
Technical Note: #8 Flexible Unit Control
Rapid and accurate estimation of protein concentration is essential in laboratory practice. Depending on the protein sample and buffers utilized, the optimal spectrophotometrical method should be performed. Direct protein UV measurements at 280 nm require pure proteins solved in buffers without absorbance interference at 280 nm. If these conditions are unable to be met, it is recommended to use a protein assay: Bradford, Biuret, BCA or Lowry. Read More…